Plasmid

Part:BBa_K1369006:Design

Designed by: Monique Alkiewicz   Group: iGEM14_Caltech   (2014-10-09)


pBAD FsrA, pFsrA-GFP, RR with no recruitment


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3656
    Illegal XbaI site found at 5291
    Illegal XbaI site found at 6977
    Illegal SpeI site found at 3662
    Illegal PstI site found at 8166
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3656
    Illegal NheI site found at 4906
    Illegal SpeI site found at 3662
    Illegal PstI site found at 8166
    Illegal NotI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3656
    Illegal BglII site found at 4929
    Illegal XhoI site found at 5679
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3656
    Illegal XbaI site found at 5291
    Illegal XbaI site found at 6977
    Illegal SpeI site found at 3662
    Illegal PstI site found at 8166
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3656
    Illegal XbaI site found at 5291
    Illegal XbaI site found at 6977
    Illegal SpeI site found at 3662
    Illegal PstI site found at 8166
    Illegal NgoMIV site found at 2275
    Illegal NgoMIV site found at 7432
    Illegal NgoMIV site found at 7715
    Illegal AgeI site found at 2435
    Illegal AgeI site found at 4690
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6692
    Illegal BsaI site found at 6870
    Illegal BsaI site found at 8803
    Illegal BsaI.rc site found at 6528
    Illegal SapI site found at 4672
    Illegal SapI site found at 5259
    Illegal SapI site found at 7655
    Illegal SapI site found at 7865


Design Notes

This does no contain the scaffold protein, so it should give us a negative result when testing the two component system.


Source

It comes from Enterococcus faecalis D32.

References